Webb19 sep. 2015 · Please add 1/10 volume reactionmixture. Since 10 bufferdoes containBSA, addBSA finalconcentration 0.01%.Some restriction enzymes 100%activity when BSA TritonX-100 reactionsystem. Since BSA TritonX-100 supplied 10concentration (0.1%), add1/10 volume reactionmixture like buffer before starting reaction.Instructions useAdd … Webb会员中心. vip福利社. vip免费专区. vip专属特权
1.12: Restriction Digest with Gel Electrophorisis
Webb25 sep. 2002 · 17.5.5 Methylation. The internal HindIII and EcoRI sites in cDNA are protected by AluI and EcoRI methylases, respectively. The cDNA pellet is collected by … Webb11 mars 2015 · 【求助】HindIII的保护碱基 RT，我现在在设计引物，往酶切位点外面加入保护碱基，但是HindIII的保护碱基就不知道加几个了，我们实验室的人说几个都可以， … half human half alligator
NEB保护碱基 - 豆丁网
WebbThermo Scientific HindIII 限制性内切酶可识别 A^AGCTT 位点，于 37°C 下在 R 缓冲液中的切割效果最佳。有关本限制性内切酶及其他限制性内 … Webb5保护碱基+酶切位点+基因20-30个碱基，如前加黑片段，设计一个F端引物跟一个R端引物 F端：5’-CcggaattcATGGTGAGCAAGGGCGAGG-3’ R端：5’-ATGGACGAGCTGTACAAGTAAggatccgcg-3’的反向互补链，我们可以借助以下网址将序列反向互补 所以引物序列就是 F端：5’-CcggaattcATGGTGAGCAAGGGCGAGG-3’ R … WebbA. 5'. Thermo Scientific BshTI (AgeI) restriction enzyme recognizes A^CCGGT sites and cuts best at 37°C in O buffer (isoschizomers: AgeI, AsiGI, CspAI, PinAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. bunbury driving school