Webb1 jan. 1999 · This permitted a Tn phoA -specific primer to be used to determine the sequence of the Agrobacterium DNA adjacent to the transposon in each plasmid. Of the sequences obtained, only those from LTU61 (in pVS1501) and LTU62 (in pVS1502) shared homology with known genes (see below). Webbaerobically. For induction of -galactosidase, 1mM (final concentration) of IPTG (isopropyl-beta-D-thioga-lactopyranoside) was added. For induction of pPRO LarA.-phoA, 1mM (final concentration) of IPTG and 10mM (final concentration) of L-Arabinose were added as required. In each culture, ampicillin (100mg/ml) and kanamycin (25mg/ml) were ...
Identification and characterization of alkaline phosphatase gene
WebbThe phoA promoter is repressed by high phosphate concentrations. Fig. 3-5-2 shows the regulation of the phoA promoter in brief. The phoA promoter is regulated by phoB and … WebbPhoA fusions confirmed that TcaA is a trans- ... Interestingly, the induction or overexpression of tcaA did not contribute further to teicoplanin susceptibility, suggesting that small amounts of TcaA, such as those present under normal uninduced conditions, were sufficient for TcaA-mediated teicoplanin susceptibility. friends in high places chords
Metabolic regulation of Escherichia coli and its phoB and phoR
Webb26 feb. 2002 · PhoA has been widely used in topology studies as it folds into an enzymatically active conformation only in the periplasm ( 22, 23 ). In contrast, GFP folds efficiently in the cytoplasm but does not form active enzyme when targeted to the periplasm by a Sec-type signal peptide ( 24 ). Webb1 okt. 2006 · Summary To examine the relationship between folding and aggregation in the periplasm of Escherichia coli, we have analysed the cellular fates of exported proteins fused to either the wild‐type maltose‐binding protein (MalE) or the aggregation‐prone variant MalE31. The propensity of fusion proteins to aggregate in the periplasm was … Webb20 sep. 2024 · PhoA and MBP fragments encompassing individual chaperone-binding sites were studied for complex formation with isolated CBD1 or CBD2 (fig. S5). PhoA sites a, b, and c bind exclusively to CBD1, and PhoA sites d and e bind exclusively to CBD2, whereas PhoA sites f and g bind either to CBD1 or CBD2 with a preference toward the latter (fig. … friends in hebrew language